Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Master Transcription Regulators02:23

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
Master Transcription Regulators02:23

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
RNA Polymerase II Accessory Proteins02:36

RNA Polymerase II Accessory Proteins

Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form dimers that...
Negative Regulator Molecules01:23

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

β-Arrestin-biased activation of type I angiotensin II receptors improves prognosis of murine pediatric heart failure.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie·2026
Same author

Pathogenic Ca<sub>V</sub>1.1 Variants Cause Hyperpolarizing Shift of Depolarization-Induced Ca<sup>2+</sup> Release in Malignant Hyperthermia Susceptibility.

Communications biology·2026
Same author

[Non-clinical safety assessments using human cells for pediatric drug development].

Nihon yakurigaku zasshi. Folia pharmacologica Japonica·2026
Same author

A hemoglobin-derived peptide, VD-hemopressin, activates hypothalamic oxytocin neurons and promotes social approach behavior in mice.

Neuroscience·2026
Same author

Immune regulation following allogeneic iPSC-derived cardiomyocyte transplantation in non-human primates.

Cardiovascular research·2025
Same author

Corrigendum to "Behavioral investigation of the sense of agency in rats by manipulating temporal delays between response and outcome: Insights from a causal reasoning task" [Behav. Brain Res. 496 (2026) 115814].

Behavioural brain research·2025

Related Experiment Video

Updated: Jul 3, 2026

Promoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions
10:16

Promoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions

Published on: June 28, 2018

Prg1 is regulated by the basic helix-loop-helix transcription factor Math2.

Misa Yamada1, Yoshiko Shida, Kou Takahashi

  • 1Department of Psychogeriatrics, National Institute of Mental Health, National Center of Neurology and Psychiatry, Tokyo, Japan.

Journal of Neurochemistry
|July 23, 2008
PubMed
Summary

Math2 directly regulates plasticity-related gene 1 (Prg1) expression, which is crucial for neurite outgrowth in PC12 cells. This Math2-Prg1 cascade is vital for neuronal differentiation and maturation.

More Related Videos

Methods to Discover Alternative Promoter Usage and Transcriptional Regulation of Murine Bcrp1
11:02

Methods to Discover Alternative Promoter Usage and Transcriptional Regulation of Murine Bcrp1

Published on: May 27, 2016

Massively Parallel Reporter Assays in Cultured Mammalian Cells
11:03

Massively Parallel Reporter Assays in Cultured Mammalian Cells

Published on: August 17, 2014

Related Experiment Videos

Last Updated: Jul 3, 2026

Promoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions
10:16

Promoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions

Published on: June 28, 2018

Methods to Discover Alternative Promoter Usage and Transcriptional Regulation of Murine Bcrp1
11:02

Methods to Discover Alternative Promoter Usage and Transcriptional Regulation of Murine Bcrp1

Published on: May 27, 2016

Massively Parallel Reporter Assays in Cultured Mammalian Cells
11:03

Massively Parallel Reporter Assays in Cultured Mammalian Cells

Published on: August 17, 2014

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Genetics

Background:

  • Math2 (NEX-1/NeuroD6) is a transcription factor involved in neuronal development.
  • Understanding Math2's regulatory targets is key to elucidating neuronal maturation processes.

Purpose of the Study:

  • To identify genes targeted by Math2.
  • To investigate the regulatory relationship between Math2 and plasticity-related gene 1 (Prg1).
  • To explore the role of the Math2-Prg1 pathway in neurite outgrowth.

Main Methods:

  • DNA microarrays to identify Math2-targeted genes.
  • Real-time quantitative PCR to confirm Prg1 expression.
  • Chromatin immunoprecipitation and reporter assays to determine direct binding and regulatory elements.
  • Cell transfection and siRNA to assess functional roles in PC12 cells.

Main Results:

  • Math2 directly binds to the promoter region of Prg1, specifically at the -E1 E-box.
  • Math2 induces Prg1 expression in cultured cells.
  • Both Math2 and Prg1 significantly promote neurite outgrowth in PC12 cells.
  • Prg1 knockdown inhibits Math2-induced morphological changes.

Conclusions:

  • Math2 directly regulates Prg1 expression.
  • The Math2-Prg1 cascade is essential for neurite outgrowth in PC12 cells.
  • This pathway plays a significant role in neuronal differentiation and maturation.