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Updated: Jul 3, 2026

Strategic Screening and Characterization of the Visual GPCR-mini-G Protein Signaling Complex for Successful Crystallization
Published on: March 16, 2020
G protein subtype specificity of rhodopsin intermediates metarhodopsin Ib and metarhodopsin II
Takefumi Morizumi1, Naoki Kimata, Akihisa Terakita
1Department of Biophysics, Graduate School of Science, Kyoto University, Kyoto, Japan.
Abstract:
Rhodopsin is one of the members of the G protein-coupled receptor family that can catalyze a GDP-GTP exchange reaction on the retinal G protein transducin (Gt) upon photon absorption. There are at least two intermediate states, meta-Ib and meta-II, which exhibit direct interaction with Gt. Meta-Ib binds to GDP-bound Gt, while meta-II forms a complex with Gt having no nucleotide, suggesting that meta-Ib is a state that initially interacts with Gt. Here we investigated whether or not meta-Ib exhibits specific interaction with G protein similar to meta-II, by examining the binding efficiencies of meta-Ib and meta-II to Gialpha and its mutants whose C-terminal 11 amino acids were replaced with those of Goalpha, Gqalpha and Gsalpha. The affinity of meta-Ib to the C-terminal 11 amino acids of Gtalpha was similar to those of Gialpha and its mutant with Goalpha's C-terminal 11 amino acids, whereas meta-II exhibited affinity to the C-terminal 11 amino acids of Gialpha mutant with Goalpha's C-terminal 11 amino acids about half of what was seen for Gtalpha and Gialpha. Both intermediates exhibited no affinity to the Gialpha mutants containing the C-terminal 11 amino acids of Gqalpha and Gsalpha. These results suggested that meta-Ib is the state that exhibits specific interaction with G protein as meta-II does, although meta-Ib exhibits a slightly lenient binding selectivity compared to that of meta-II.
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