Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Pleiotropy01:33

Pleiotropy

Pleiotropy is the phenomenon in which a single gene impacts multiple, seemingly unrelated phenotypic traits. For example, defects in the SOX10 gene cause Waardenburg Syndrome Type 4, or WS4, which can cause defects in pigmentation, hearing impairments, and an absence of intestinal contractions necessary for elimination. This diversity of phenotypes results from the expression pattern of SOX10 in early embryonic and fetal development. SOX10 is found in neural crest cells that form melanocytes,...
General Transcription Factors01:30

General Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
Epistasis01:39

Epistasis

In addition to multiple alleles at the same locus influencing traits, numerous genes or alleles at different locations may interact and influence phenotypes in a phenomenon called epistasis. For example, rabbit fur can be black or brown depending on whether the animal is homozygous dominant or heterozygous at a TYRP1 locus. However, if the rabbit is also homozygous recessive at a locus on the tyrosinase gene (TYR), it will have an unshaded coat that appears white, regardless of its TYRP1...
Transcription Factors02:16

Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Multivalence Aptamers Enhance Transmembrane Efficiency of Localized Catalytic Hairpin Assembly-Based Nanomachine for Sensitive Cell Imaging.

Analytical chemistry·2026
Same author

Tetrahedron DNA-encapsulated gold nanoparticles inhibit nanolayer aggregation on Ω-shaped fiber-optic surface for LSPR-based biosensor with merit of molar ratio-dependent sensitivity.

Biosensors & bioelectronics·2026
Same author

Barley HvBODYGUARD1 controls cuticular specialisations regulated by SHINE transcription factors.

The New phytologist·2026
Same author

TFAM promotes mitochondrial division by increasing mitochondrial Sirt3.

Cell death & disease·2026
Same author

OzBarley: A genetic and phenotypic data resource capturing the Australian barley breeding history.

Scientific data·2026
Same author

Integration of Dual Functions of Cytosensing and Highly Efficient Photothermal Therapy Using a Cascaded Ω-Shaped Fiber Optic Localized Surface Plasmon Resonance Sensor.

ACS sensors·2026

Related Experiment Video

Updated: Jul 3, 2026

Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput
10:29

Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput

Published on: March 30, 2018

Tissue-dependent limited pleiotropy affects gene expression in barley.

Elena Potokina1, Arnis Druka1, Zewei Luo1

  • 1School of Biosciences, The University of Birmingham, Birmingham B15 2TT, UK,Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, UK,Laboratory of Population & Quantitative Genetics, School of Life Sciences, Institute of Biomedical Sciences, Fudan University, Shanghai 200433, China,Bioinformatics and Computational Biology Graduate Program & Department of Plant Pathology, Iowa State University, Ames, IA 50011-1020, USA, andCorn Insects and Crop Genetics Research, USDA-ARS, Iowa State University, Ames, IA 50011-1020, USA.

The Plant Journal : for Cell and Molecular Biology
|July 23, 2008
PubMed
Summary

Limited pleiotropy of cis-regulatory mutations in barley (Hordeum vulgare L.) was investigated. These mutations can affect gene expression differently across tissues and developmental stages, impacting genetic studies.

More Related Videos

Investigating Tissue- and Organ-specific Phytochrome Responses using FACS-assisted Cell-type Specific Expression Profiling in Arabidopsis thaliana
10:10

Investigating Tissue- and Organ-specific Phytochrome Responses using FACS-assisted Cell-type Specific Expression Profiling in Arabidopsis thaliana

Published on: May 29, 2010

Related Experiment Videos

Last Updated: Jul 3, 2026

Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput
10:29

Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput

Published on: March 30, 2018

Investigating Tissue- and Organ-specific Phytochrome Responses using FACS-assisted Cell-type Specific Expression Profiling in Arabidopsis thaliana
10:10

Investigating Tissue- and Organ-specific Phytochrome Responses using FACS-assisted Cell-type Specific Expression Profiling in Arabidopsis thaliana

Published on: May 29, 2010

Area of Science:

  • Genomics
  • Plant Biology
  • Molecular Genetics

Background:

  • Cis-regulatory mutations can have spatially or temporally limited effects, a phenomenon known as limited pleiotropy.
  • Understanding these effects is crucial for interpreting gene expression patterns and genetic variation.

Purpose of the Study:

  • To investigate the genome-wide occurrence and characteristics of limited pleiotropy of cis-regulatory mutations in barley (Hordeum vulgare L.).
  • To identify cis-acting expression regulators and analyze their behavior across different tissues and developmental stages.

Main Methods:

  • Genome-wide analysis of 22,840 genes in 139 doubled haploid lines of barley using Affymetrix arrays.
  • Comparison of gene expression in germinating embryo tissues and seedling leaves from the embryonic axis.

Main Results:

  • Identified robust cis-acting expression regulators that segregate as major genes in both germinating embryo and seedling leaf tissues.
  • Observed that the effects of cis-regulatory polymorphisms can be tissue-specific, with parental alleles influencing expression differently across tissues.
  • Demonstrated that the number of discovered cis expression quantitative trait loci (cis-eQTLs) is significantly influenced by the studied tissue or developmental stage due to limited pleiotropy.

Conclusions:

  • Limited pleiotropy is a common feature of cis-regulatory mutations in barley.
  • This phenomenon has significant implications for genetical genomics studies, as findings are highly dependent on the chosen tissue and developmental stage.
  • The principles of limited pleiotropy are likely applicable to tissue-specific gene regulation across a broad range of plant and animal species.