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Real-time protein biosensor arrays based on surface plasmon resonance differential phase imaging
1Department of Electronic Engineering, The Chinese University of Hong Kong, Hong Kong, Hong Kong, China.
Biosensors & Bioelectronics
|July 23, 2008
Summary
This study introduces a novel differential phase surface plasmon resonance (SPR) imaging technique for high-density, two-dimensional protein biosensor arrays. This method enhances detection resolution and enables real-time, label-free protein analysis for applications in diagnostics and drug screening.
Area of Science:
- Biotechnology
- Analytical Chemistry
- Surface Science
Background:
- Conventional angular Surface Plasmon Resonance (SPR) imaging is limited to one-dimensional arrays due to detector requirements.
- High-density two-dimensional (2D) biosensor arrays are crucial for high-throughput analysis in various biological applications.
Purpose of the Study:
- To develop and demonstrate a differential phase SPR imaging technique for 2D protein biosensor arrays.
- To improve the utilization efficiency and detection resolution of SPR biosensor systems.
- To enable real-time, label-free monitoring of biomolecular interactions.
Main Methods:
- Application of differential phase SPR imaging to 2D protein biosensor arrays.
- Utilizing phase-sensitive SPR to capture data in the time domain, allowing each pixel to represent a sensor site.
- Implementing common-mode disturbance removal for enhanced detection resolution.
Main Results:
- Achieved a system resolution of 8.8 x 10(-7) refractive index unit (RIU).
- Demonstrated real-time monitoring of bovine serum albumin (BSA)/anti-BSA binding interactions.
- Established a detection limit of 0.77 microg/ml for BSA/anti-BSA interactions.
Conclusions:
- The differential phase SPR imaging technique enables high-density 2D protein biosensor arrays.
- This approach offers a real-time, non-labeling detection tool for high-throughput protein array analysis.
- Potential applications include protein therapeutics, drug screening, and clinical diagnostics.
