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A Protocol for Analyzing Hepatitis C Virus Replication
Published on: June 26, 2014
Synthesis and evaluation of 5'-modified 2'-deoxyadenosine analogues as anti-hepatitis C virus agents
Masahiro Ikejiri1, Takayuki Ohshima, Akemi Fukushima
1Faculty of Pharmaceutical Sciences at Kagawa Campus, Tokushima Bunri University, 1314-1 Shido, Sanuki, Kagawa 769-2193, Japan. ikejim@osaka-ohtani.ac.jp
Abstract:
In order to study the effect of 5'-modification of 2'-deoxynucleoside on its anti-HCV activity, several analogues were synthesized and evaluated. Among the analogues, a 5'-deoxy-5'-phenacylated analogue exhibited a good anti-HCV activity with an EC(50) of 15.1 microM. This compound is expected to operate via a type of mechanism that does not involve a generally known 5'-O-triphosphorylation process.
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