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Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Next-generation Sequencing03:00

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.
Sanger Sequencing01:57

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...

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DNAzyme 10-23 - Based Nanomachines for Nucleic Acid Recognition
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DNAzyme 10-23 - Based Nanomachines for Nucleic Acid Recognition

Published on: February 9, 2024

Recent advances in DNA sensors.

Serge Cosnier1, Pascal Mailley

  • 1Département de Chimie Moléculaire, UMR-5250, ICMG FR-2607, CNRS, Université Joseph Fourier BP 53, 38041 Grenoble Cédex 9, France. serge.cosnier@ujf-grenoble.fr

The Analyst
|July 23, 2008
PubMed
Summary

DNA biosensors offer rapid, sensitive genetic detection. Recent advances focus on improving probe immobilization and detection strategies for enhanced DNA analysis.

Area of Science:

  • Biochemistry
  • Surface Chemistry
  • Molecular Electrochemistry
  • Micro-technologies
  • Optics
  • Electronics

Background:

  • DNA biosensors are crucial for rapid and sensitive genetic detection.
  • Their development requires interdisciplinary expertise, including surface physics, chemistry, and biochemistry.
  • Key steps involve probe immobilization, hybridization, and signal reading.

Purpose of the Study:

  • To review recent advancements in DNA biosensor technology.
  • Focusing on improvements in probe immobilization and detection strategies.
  • Highlighting the integration of reading points and strategies for enhanced performance.

Main Methods:

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Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules
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Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules

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Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules

Published on: June 1, 2011

  • Review of recent scientific literature on DNA biosensor development.
  • Focus on techniques for oligonucleotide probe immobilization on substrates.
  • Analysis of novel detection and reading strategies for hybridized DNA.

    • Main Results:

    • Progress in surface functionalization for efficient probe immobilization.
    • Development of integrated reading points and advanced detection strategies.
    • Enhanced sensitivity and speed in genetic analysis using DNA biosensors.

    Conclusions:

    • Recent advances significantly improve DNA biosensor capabilities.
    • Integration of immobilization and detection steps is key to performance.
    • DNA biosensors are vital tools for genetic detection across various fields.