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[In vitro cytotoxicity and oxidative damage effects of multi-wall carbon nanotube on RAW264.7 macrophages]
Ying Liu1, Weimin Song, Weihua Li
1School of Public Health, Fudan University, Shanghai 200032, China.
Objective:
To study the in vitro cytotoxicity and oxidative damages of multi-wall carbon nanotube (MWCNTs) on RAW264.7 macrophages.
Methods:
The RAW264.7 macrophages were treated with different concentrations of MWCNTs (2.5, 10, 25 and 100 microg/ml), deoxyribonucleic acid sodium salt and saline for 24h. deoxyribonucleic acid sodium salt were used for increasing the dispersity of MWCNTs. MTT assays were applied to testing the viability of RAW264.7 cell. The contents of Total protein (TP), nitrogen monoxidum (NO), glutathione (GSH) and malondialdehyde (MDA) ,the activities of superoxide dismutase (SOD) and lactate dehydrogenase (LDH), were determined in supernate and intracellular. The morphological change of RAW 264.7 were also observed after exposure to MWCNTs for 24h.
Results:
Compared with the controls, significant differences in the viability, cytotoxicity and oxidative damages were observed in 25 and 100 microg/ml groups. With the administration concentrations increased, intracellular or extracelluar levels of TP, LDH, NO and MDA in treatment groups increased, while the levels of GSH and SOD decreased with a significant dose-effect relationship. Which accord to the morphological change of RAW 264.7 after exposure to MWCNTs for 24h.
Conclusion:
MWCNTs could cause cytotoxicity and oxidative damages to RAW264.7 macrophages, with a dose-effect relationship.
Insights
Multi-wall carbon nanotubes (MWCNTs) induce cytotoxicity and oxidative stress in RAW264.7 macrophages. Higher MWCNT concentrations led to increased cell damage and altered biochemical markers, demonstrating a clear dose-effect relationship.
Area of Science:
- Nanomaterial toxicology
- Cellular biology
- Oxidative stress research
Context:
- Macrophage cells (RAW264.7) are crucial immune responders.
- Carbon nanotubes (CNTs) are widely used nanomaterials with potential biological interactions.
- Understanding nanotoxicology is vital for safe application of novel materials.
Purpose:
- To investigate the in vitro cytotoxicity of multi-wall carbon nanotubes (MWCNTs).
- To assess MWCNT-induced oxidative damages in RAW264.7 macrophages.
- To establish a dose-response relationship for MWCNT effects on macrophages.
Summary:
- RAW264.7 macrophages were exposed to varying concentrations of MWCNTs (2.5-100 µg/mL).
- Cell viability, total protein, nitric oxide, glutathione, malondialdehyde, superoxide dismutase, and lactate dehydrogenase were measured.
- Morphological changes and biochemical markers indicated significant cytotoxicity and oxidative damage at higher MWCNT concentrations (25 and 100 µg/mL).
Impact:
- Demonstrates MWCNTs induce dose-dependent cytotoxicity and oxidative stress in macrophages.
- Provides insights into the cellular mechanisms of MWCNT toxicity.
- Informs risk assessment for MWCNT exposure in biological systems.
