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Published on: November 8, 2018
[Noninvasive fetal sex detection from maternal plasma in pregnant women]
R Vodicka1, R Vrtĕl, E Schneiderová
1Ustav lékarské genetiky a fetální medicíny, Fakultnńemocnice Olomouc.
Objective:
Objective of our study was optimization of noninvasive fetal sex detection from maternal plasma in pregnant women.
Study Design:
Molecular DNA quantitative analyses in gonosomal loci.
Setting:
The study was performed at Department of Medical Genetics and Fetal Medicine, University Hospital Olomouc.
Methods:
Together 475 DNA samples isolated from maternal plasma in different weeks of pregnancy ranging from 4th w.g. to 37th w.g. Y chromosomal sequences in AMELY and TSPY were tested by refined quantitative fluorescent PCR using capillary electrophoresis.
Results:
The method is able to distinguish 1% of Y chromosomal sequences of artificial mixtures. Investigation and assessment in cell free fetal DNA samples achieved 4.05% of false positivity and 7.15% of false negativity in Y sequence detection.
Conclusion:
Established method allows detecting fetal sex with high sensitivity and specificity. The method is possible to use also for quantitative purposes.
