Related Experiment Video
Updated: Jul 3, 2026
Development of Compendium for Esophageal Squamous Cell Carcinoma
Published on: April 12, 2024
[Expression of nucleostemin mRNA and protein in the esophageal squamous cell carcinoma]
Gong-Yuan Zhang1, Lei Yin, Sheng-Lei Li
1Department of Histology and Embryology, College of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450052, China.
Objective:
To investigate the mRNA and protein expression of nucleostemin (NS) in human esophageal squamous cell carcinoma.
Methods:
The mRNA and protein expression of NS were detected in 31 mucosal atypical hyperplasia specimens, 62 esophageal squamous cell carcinoma specimens and the matched normal esophageal mucosa samples by RT-PCR and immunohistochemistry method, respectively.
Results:
The positive expression rate of NS protein in normal esophageal mucosa, atypical hyperplasia and esophageal squamous cell carcinoma was 17.7% (11/62), 41.9% (13/31) and 69.4% (43/62), respectively. There was a significant difference among the above three groups (chi2 = 33.676, P < 0.01). The expression levels of NS mRNA in esophageal squamous cell carcinoma (0.971 +/- 0.121) was significantly higher than that in the atypical hyperplasia (0.913 +/- 0.085) and also in the normal esophageal mucosa (0.866 +/- 0.103; F = 14.829, P < 0.01). The expression level of both NS protein and mRNA was positively correlated with histological grade, infiltration depth, and lymph node metastasis (P < 0.05), but not with age, gender or pathological type (P > 0.05).
Conclusion:
Our results indicate that nucleostemin mRNA and protein are over-expressed in human esophageal squamous cell carcinoma, and it may be related with its oncogenesis.
