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Related Experiment Videos

Major histocompatibility complex antigen expression on lymphocytes from aging strain A mice.

D Janick-Buckner1, C J Briggs, T E Meyer

  • 1Department of Biology, Northeastern University, Boston, MA 02115.

Growth, Development, and Aging : GDA
|January 1, 1991
PubMed
Summary

Major histocompatibility complex (MHC) class I antigen levels increase with age in mice, while class II levels decrease. This age-related shift in MHC expression impacts immune cell populations and function.

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Area of Science:

  • Immunology
  • Molecular Biology
  • Aging Research

Background:

  • Major histocompatibility complex (MHC) molecules are critical for immune system function.
  • MHC antigens present peptides to T cells, initiating immune responses.
  • Age-related changes in immune function are well-documented, but molecular underpinnings require further study.

Purpose of the Study:

  • To investigate age-dependent alterations in MHC class I and class II antigen expression on lymphocytes in strain A mice.
  • To correlate changes in MHC protein levels with corresponding mRNA expression.
  • To understand how these changes affect lymphocyte populations, particularly B cells and T cells.

Main Methods:

  • Quantification of MHC class I and class II protein levels on peripheral blood and spleen lymphocytes using flow cytometry.

Related Experiment Videos

  • Measurement of MHC class I and class II mRNA levels via quantitative analysis.
  • Analysis of spleen lymphocyte populations to determine the relative proportions of B cells and T cells.

    • Main Results:

    • MHC class I protein and mRNA levels significantly increased with age in both peripheral blood and spleen lymphocytes.
    • MHC class II protein levels showed no significant age-related change, but mRNA levels decreased markedly.
    • The percentage of MHC class II-expressing spleen lymphocytes decreased with age due to a reduced proportion of B cells.

    Conclusions:

    • MHC class I and class II gene expression regulation exhibits distinct age-dependent patterns in strain A mice.
    • Changes in MHC expression with aging may impact the efficacy of cellular and humoral immune responses.
    • Further research is needed to explore the modulation of MHC gene expression in aged individuals to potentially restore immune function.