Identification of the coat protein gene of bean golden mosaic geminivirus
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A Murayama1, Y Yamaya, M Ikegami
1NODAI Research Institute, Tokyo University of Agriculture, Japan.
Intervirology
|January 1, 1991
Abstract:
An open-reading frame designated 1R1, on DNA component 1 of bean golden mosaic geminivirus (BGMV), has been identified as the coat protein gene. A DraI restriction fragment of BGMV DNA1 that includes 1R1 was inserted into the SmaI pKK223-3 expression vector. The 32 kD protein expressed in Escherichia coli cells reacted with antibodies to the BGMV capsid polypeptide and behaved identically to purified capsid protein in western blots.