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Imaging Biological Samples with Optical Microscopy

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...

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Related Experiment Video

Updated: Jul 3, 2026

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy

Published on: July 25, 2022

High-throughput, multiplexed pushbroom hyperspectral microscopy.

M E Gehm¹, M S Kim, C Fernandez

¹University of Arizona Electrical and Computer Engineering Department, Tucson, AZ 85721, USA.

|July 24, 2008

Summary

This summary is machine-generated.

A new hyperspectral microscope uses a coded aperture mask, achieving 32x higher throughput than conventional systems. This innovation offers 1 nm spectral resolution for advanced imaging applications.

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Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals

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Published on: August 22, 2019

Area of Science:

Optics and Photonics
Microscopy
Spectroscopy

Background:

Conventional pushbroom spectral imagers utilize a slit, limiting throughput.
Hyperspectral microscopy requires high spectral resolution and throughput for detailed analysis.

Purpose of the Study:

To introduce a novel high-throughput hyperspectral microscope design.
To demonstrate the theoretical and experimental feasibility of aperture-coded spectral imaging.

Main Methods:

Replaced the conventional slit with a static coded aperture mask in a hyperspectral microscope.
Developed and tested two proof-of-concept experimental implementations of the aperture-coded spectral engine.
Characterized spectral resolution (approx. 1 nm) and spatial resolution (ranging from 7.7 to 1.54 micrometers).

Main Results:

The aperture-coded systems demonstrated 32 times greater throughput compared to conventional push broom systems.
Achieved a spectral resolution of approximately 1 nm across the 550-665 nm spectral range.
Two distinct system designs exhibited varying spatial resolutions, suitable for different microscopic applications.

Conclusions:

The aperture-coded spectral engine offers a significant throughput enhancement for hyperspectral microscopy.
The developed systems provide high spectral resolution, enabling detailed analysis in proof-of-concept applications.
This technology represents a promising advancement for high-throughput hyperspectral imaging in scientific research.