Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...
Subcellular Fractionation01:32

Subcellular Fractionation

The homogenate obtained after cell lysis contains various membrane-bound organelles that can be further separated into pure fractions by subcellular fractionation. These isolates are used to study specific cellular components, analyze localized protein activity, and are even employed in diagnostics. Fractionation is typically achieved using centrifugation methods, the most common being density-gradient and differential centrifugation.
Differential Centrifugation
Differential centrifugation is...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Stem Cell Mobilization and Autologous Transplantation Outcomes After First-Line R-DA-EPOCH in High-Risk DLBCL and HGBCL: A Single-Center Retrospective Study.

Cancers·2026
Same author

Mesenchymal stem cells and their extracellular vesicles: a new therapeutic landscape in bronchopulmonary dysplasia.

Thorax·2026
Same author

Pre-treatment circulating microRNA signatures predict outcomes of anti-CD19 CAR T-cell therapy.

Haematologica·2026
Same author

Metabolic syndrome in cushing's syndrome patients: Does gender matter?

Pituitary·2026
Same author

TRKB-based signature identifies high-risk squamous cell carcinoma cases and TRKB blockade reprograms tumor and stromal cells toward suppressive phenotypes.

Journal of biomedical science·2026
Same author

Label-free optical fingerprints of hyperoxia-induced lung alterations in preterm rabbits.

Respiratory research·2026

Related Experiment Video

Updated: Jul 3, 2026

Flow-sorting and Exome Sequencing of the Reed-Sternberg Cells of Classical Hodgkin Lymphoma
08:53

Flow-sorting and Exome Sequencing of the Reed-Sternberg Cells of Classical Hodgkin Lymphoma

Published on: June 10, 2017

Human lymphocyte sorting by gravitational field-flow fractionation.

Barbara Roda1, Pierluigi Reschiglian, Andrea Zattoni

  • 1Department of Chemistry "G. Ciamician", University of Bologna, Via Selmi 2, 40126, Bologna, Italy. barbara.roda@unibo.it

Analytical and Bioanalytical Chemistry
|July 24, 2008
PubMed
Summary

Gravitational field-flow fractionation (GrFFF) effectively sorts human lymphocytes, separating cancerous B cells from healthy ones. This method achieves high depletion of neoplastic cells without fluorescent tags, crucial for cancer treatments.

More Related Videos

Isolation of Human Lymphatic Endothelial Cells by Multi-parameter Fluorescence-activated Cell Sorting
07:36

Isolation of Human Lymphatic Endothelial Cells by Multi-parameter Fluorescence-activated Cell Sorting

Published on: May 1, 2015

Immunophenotyping and Cell Sorting of Human MKs from Human Primary Sources or Differentiated In Vitro from Hematopoietic Progenitors
14:30

Immunophenotyping and Cell Sorting of Human MKs from Human Primary Sources or Differentiated In Vitro from Hematopoietic Progenitors

Published on: August 7, 2021

Related Experiment Videos

Last Updated: Jul 3, 2026

Flow-sorting and Exome Sequencing of the Reed-Sternberg Cells of Classical Hodgkin Lymphoma
08:53

Flow-sorting and Exome Sequencing of the Reed-Sternberg Cells of Classical Hodgkin Lymphoma

Published on: June 10, 2017

Isolation of Human Lymphatic Endothelial Cells by Multi-parameter Fluorescence-activated Cell Sorting
07:36

Isolation of Human Lymphatic Endothelial Cells by Multi-parameter Fluorescence-activated Cell Sorting

Published on: May 1, 2015

Immunophenotyping and Cell Sorting of Human MKs from Human Primary Sources or Differentiated In Vitro from Hematopoietic Progenitors
14:30

Immunophenotyping and Cell Sorting of Human MKs from Human Primary Sources or Differentiated In Vitro from Hematopoietic Progenitors

Published on: August 7, 2021

Area of Science:

  • Biomedical Engineering
  • Cell Biology
  • Cancer Research

Background:

  • Increasing demand for cell sorting in biomedical applications, from research to clinical treatments.
  • Critical need for precise discrimination and quantification of distinct cell subpopulations.
  • Importance of neoplastic cell depletion in autologous transplantation for blood cancer therapies.

Purpose of the Study:

  • To demonstrate the efficacy of gravitational field-flow fractionation (GrFFF) for sorting heterogeneous human lymphocytes.
  • To separate neoplastic B cells from a Burkitt lymphoma cell line and healthy T and B lymphocytes.
  • To evaluate GrFFF as a label-free cell sorting technique for biomedical applications.

Main Methods:

  • Utilized gravitational field-flow fractionation (GrFFF) to process a mixture of human lymphocytes.
  • Employed flow cytometry for assessing cell viability and evaluating the achieved cell fractionation.
  • Conducted a clonogenicity test to confirm the depletion of neoplastic cells.

Main Results:

  • GrFFF successfully sorted a heterogeneous mixture of human lymphocytes, including neoplastic B cells and healthy T and B lymphocytes.
  • The method achieved a high depletion of neoplastic cells, with less than 2% found in a specific fraction.
  • Sorted cells retained viability and could be collected for further characterization.

Conclusions:

  • Gravitational field-flow fractionation (GrFFF) is an effective label-free technique for sorting viable human lymphocytes.
  • GrFFF enables high depletion of neoplastic cells, showing promise for applications like autologous transplantation in cancer treatment.
  • The method allows for the collection and further analysis of sorted cell populations.