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Published on: May 1, 2020
The human Y-encoded testis-specific protein interacts functionally with eukaryotic translation elongation factor
Tatsuo Kido1, Yun-Fai Chris Lau
1Division of Cell and Developmental Genetics, Department of Medicine, Veterans Affairs Medical Center, University of California, San Francisco, CA 94121, USA.
International Journal of Cancer
|July 24, 2008
Summary
Testis-specific protein Y-encoded (TSPY) interacts with translation elongation factor eEF1A. This interaction stimulates gene expression and protein synthesis, suggesting TSPY
Area of Science:
- Molecular Biology
- Oncology
- Genetics
Background:
- Testis-specific protein Y-encoded (TSPY) is implicated in gonadoblastoma and expressed in various cancers.
- TSPY belongs to the TSPY/SET/NAP1 superfamily, featuring a conserved SET/NAP domain.
Purpose of the Study:
- To investigate the role of TSPY in tumorigenesis by identifying its binding partners.
- To explore the functional interaction between TSPY and translation elongation factor eEF1A.
Main Methods:
- Yeast two-hybrid screen of a fetal gonadal cDNA library to identify TSPY binding partners.
- Co-immunoprecipitation and immunofluorescence in COS7 cells and human seminoma specimens to confirm TSPY-eEF1A interaction.
- Reporter gene assays to assess the impact of TSPY and eEF1A on protein synthesis and gene transcription.
Main Results:
- TSPY was identified to bind with translation elongation factor eEF1A at the SET/NAP domain.
- TSPY and eEF1A were found to be highly expressed and colocalized in tumor germ cells of human seminoma.
- TSPY enhanced reporter gene expression, an effect amplified by eEF1A overexpression, and promoted nuclear redistribution of eEF1A.
Conclusions:
- TSPY interacts with eEF1A, potentially contributing to its oncogenic functions.
- The TSPY-eEF1A interaction stimulates both protein synthesis and gene transcription, suggesting a role in cancer development.
- Targeting the TSPY-eEF1A interaction could offer new therapeutic strategies for TSPY-associated cancers.
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