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Related Experiment Video

Updated: Jul 3, 2026

Three-Dimensional Cell Culture of Adipose-Derived Stem Cells in a Hydrogel with Photobiomodulation Augmentation
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Three-Dimensional Cell Culture of Adipose-Derived Stem Cells in a Hydrogel with Photobiomodulation Augmentation

Published on: April 5, 2024

Stem cell proliferation under low intensity laser irradiation: a preliminary study.

Fernanda de P Eduardo1, Daniela F Bueno, Patricia M de Freitas

  • 1Hospital Israelita Albert Einstein, Unit of Bone Marrow Transplantation, São Paulo 05651-901, SP, Brazil.

Lasers in Surgery and Medicine
|July 24, 2008
PubMed
Summary

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Low-level laser therapy enhances human dental pulp stem cell (hDPSC) proliferation, particularly under nutrient-deprived conditions. This finding suggests potential applications in regenerative medicine and tissue engineering.

Area of Science:

  • Biomedical Engineering
  • Stem Cell Biology
  • Regenerative Medicine

Background:

  • Low-intensity laser irradiation is known to promote cell proliferation.
  • Human dental pulp stem cells (hDPSCs) are crucial for dental tissue regeneration.

Purpose of the Study:

  • To investigate the effect of 660 nm laser phototherapy on hDPSC proliferation in vitro.
  • To assess if laser irradiation can enhance cell growth under nutrient-deficient conditions.

Main Methods:

  • hDPSCs were cultured under nutritional deficit (10% FBS).
  • Cells were irradiated using a 660 nm InGaAIP diode laser at 20 mW/6s and 40 mW/3s settings.
  • Cell proliferation was measured via MTT assay to assess mitochondrial activity.

Main Results:

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Last Updated: Jul 3, 2026

Three&#45;Dimensional Cell Culture of Adipose-Derived Stem Cells in a Hydrogel with Photobiomodulation Augmentation
05:42

Three-Dimensional Cell Culture of Adipose-Derived Stem Cells in a Hydrogel with Photobiomodulation Augmentation

Published on: April 5, 2024

Laser Microirradiation to Study In Vivo Cellular Responses to Simple and Complex DNA Damage
10:44

Laser Microirradiation to Study In Vivo Cellular Responses to Simple and Complex DNA Damage

Published on: January 31, 2018

Control of Cell Geometry through Infrared Laser Assisted Micropatterning
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Control of Cell Geometry through Infrared Laser Assisted Micropatterning

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  • The 20 mW laser setting significantly increased MTT activity at 72 hours compared to controls.
  • Irradiated cultures under nutritional deficit showed significantly higher viable cells after 24 hours than non-irradiated cultures.
  • Laser phototherapy at 20 mW demonstrated a positive effect on hDPSC growth.

Conclusions:

  • hDPSCs exhibit a positive response to laser phototherapy, with improved cell growth under nutrient-deficient conditions.
  • Laser phototherapy combined with hDPSCs holds promise for future tissue engineering and regenerative medicine applications.
  • The findings suggest potential for laser phototherapy to enhance the growth of other stem cell types.