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Updated: Jul 3, 2026
ELIME (Enzyme Linked Immuno Magnetic Electrochemical) Method for Mycotoxin Detection
Published on: October 23, 2009
Development of a selective sample clean-up method based on immuno-ultrafiltration for the determination of
Christina Böhm1, Margit Cichna-Markl, Zdenka Brenn-Struckhofova
1Department of Veterinary Public Health and Food Science, Institute of Nutrition, University of Veterinary Medicine, Veterinärplatz 1, A-1210 Vienna, Austria.
Abstract:
This paper describes the development of a highly selective analytical method for the determination of deoxynivalenol (DON) in maize. The developed method is based on immuno-ultrafiltration (IUF) and is the first application of IUF as a clean-up strategy in food analysis. Quantification of DON was carried out by high-performance liquid chromatography with ultraviolet detection. In contrast to immunoaffinity chromatography, in IUF the antibodies are not bound to a solid support material but used in free form, thus making it possible to avoid the critical immobilisation step. Sample clean-up by IUF proved to be as selective as clean-up using commercially available immunoaffinity columns. The limit of detection (S/N=3) of the analytical method was found to be 74 ng DON/g maize. Repeated analysis of a certified maize reference material on four different days resulted in a mean recovery of 93% with a standard deviation of 10%.
