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Updated: Jul 3, 2026

Method for the Isolation of Francisella tularensis Outer Membranes
Published on: June 29, 2010
Molecular method for discrimination between Francisella tularensis and Francisella-like endosymbionts
Raquel Escudero1, A Toledo, Horacio Gil
1Laboratorio de Espiroquetas y Patógenos Especiales, Servicio de Bacteriología, Centro Nacional de Microbiología, Majadahonda, Madrid, Spain.
Abstract:
Environmental studies on the distribution of Francisella spp. are hampered by the frequency of Francisella-like endosymbionts that can produce a misleading positive result. A new, efficient molecular method for detection of Francisella tularensis and its discrimination from Francisella-like endosymbionts, as well as two variants associated with human disease (unusual F. tularensis strain FnSp1 and F. tularensis subsp. novicida-like strain 3523), is described. The method is highly specific and sensitive, detecting up to one plasmid copy or 10 genome equivalents.
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