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Published on: September 2, 2019
The first doubled haploid linkage map for cultivated oat.
Pirjo Tanhuanpää1, Ruslan Kalendar, Alan H Schulman
1Plant Genomics, Biotechnology and Food Research, MTT Agrifood Research Finland, FI-31600 Jokioinen, Finland. pirjo.tanhuanpaa@mtt.fi
Genome
|July 25, 2008
Summary
A new doubled haploid (DH) linkage map for hexaploid oat (Avena sativa L.) was created using 625 DNA markers. This map aids in localizing genes for important agronomic and quality traits.
Area of Science:
- * Plant genetics and genomics
- * Crop science and breeding
Background:
- * Recombinant inbred lines (RILs) have traditionally been used for hexaploid oat linkage map construction.
- * Doubled haploids (DHs) offer superior homozygosity, but large DH populations were recently enabled in oats.
- * This study leverages DH technology for a more comprehensive oat genetic map.
Purpose of the Study:
- * To construct a high-density linkage map for hexaploid oat (Avena sativa L.) using a doubled haploid population.
- * To establish a genetic framework for quantitative trait loci (QTL) analysis of agronomic and quality traits in oat.
- * To explore marker segregation distortion in the developed oat map.
Main Methods:
- * Development of an anther culture-derived doubled haploid (DH) population of 137 individuals from a cross between 'Aslak' and 'Matilda' cultivars.
- * Genotyping using a diverse set of 625 DNA markers, including Amplified Fragment Length Polymorphisms (AFLPs), Inter-Retrotransposon Amplified Polymorphisms (IRAPs), Inter Simple Sequence Repeats (ISSRs), microsatellites, Random Amplified Polymorphic DNAs (RAPDs), Retrotransposon-Microsatellite Amplified Polymorphisms (REMAPs), Sequence-Related Amplified Polymorphisms (SRAPs), and Single Nucleotide Polymorphisms (SNPs).
- * Construction of a 28-linkage group map spanning 1526 cM.
Main Results:
- * A comprehensive linkage map comprising 28 linkage groups and 625 DNA markers was successfully constructed.
- * Over 50% of the markers exhibited distorted segregation, with a prevalence of alleles from the 'Aslak' cultivar, attributed to its superior anther culture performance.
- * The map provides a foundation for identifying quantitative trait loci (QTLs) associated with key oat quality and agronomic characteristics.
Conclusions:
- * The developed doubled haploid (DH) linkage map represents a significant advancement for hexaploid oat (Avena sativa L.) genetics.
- * This genetic map facilitates future research into the genetic basis of important oat traits.
- * Segregation distortion analysis provides insights into the biological factors influencing DH development in oat breeding programs.
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