Related Experiment Video
Updated: Jul 3, 2026

Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification
Published on: September 21, 2011
Cholesteric bonded stationary phases for high-performance liquid chromatography: a comparative study of the
Cédric Courtois1, Guilhem Pagès, Stefano Caldarelli
1Ism2, Aix Marseille Université, Case 512, Campus de Saint Jérôme, Avenue Escadrille Normandie-Niemen, 13397, Marseille Cedex 20, France. cedric.courtois@univ-cezanne.fr
Abstract:
The properties of four cholesteric bonded stationary phases differing in the nature of the spacer and the end-capping were assessed using simple chromatographic tests based on the retention of nonpolar compounds and of planar or nonplanar probe solutes. All cholesteric columns showed a hydrophobicity close to that of conventional octadecyldimethylsilyl (ODS) materials. Non-end-capped cholesteric bonded phases showed greater selectivity than ODS ones and both end-capped cholesteric bonded phases exhibit behavior intermediate between that of the non-end-capped original material and that of the ODS bonded phase.
Related Concept Videos
High-Performance Liquid Chromatography: Introduction
In HPLC, two phases play a critical role in the separation process:
Gas Chromatography: Types of Columns and Stationary Phases
For an analyte to remain on the column for a sufficient amount of time, it must exhibit some level of compatibility (or...
Chromatography: Introduction
The phase in which the compounds linger or on which the compounds adsorb is called the stationary phase, whereas the mobile phase is the solvent that carries the solutes to be analyzed. In traditional column chromatography, the mixture flows through the stationary phase, and the compounds partition between the stationary and mobile phases...
High-Performance Liquid Chromatography: Instrumentation
High-Performance Liquid Chromatography: Elution Process
Ion-Exchange Chromatography
