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[Comparison of two Lactobacillus strains isolated from piglets in different area by using representational difference
1Laboratory of Gastrointestinal Microbiology, Nanjing Agricultural University, Nanjing 210095, China. suney7912@yahoo.com
Wei Sheng Wu Xue Bao = Acta Microbiologica Sinica
|July 26, 2008
Summary
A piglet intestinal bacterium, Lactobacillus sp. strain S1, was identified as Lactobacillus sobrius. Genomic analysis revealed no differences between strain S1 and the known Lactobacillus sobrius 001T, indicating they are the same strain.
Area of Science:
- Microbiology
- Genomics
- Bacterial Identification
Background:
- Lactobacillus species are crucial in animal gut health.
- Accurate identification and genomic comparison of bacterial strains are essential for understanding their roles.
Purpose of the Study:
- To identify Lactobacillus sp. strain S1 isolated from piglet intestine.
- To compare the genome of strain S1 with Lactobacillus sobrius 001T.
Main Methods:
- 16S rRNA gene sequencing for bacterial identification.
- Species-specific polymerase chain reaction (PCR) assays.
- Representational difference analysis (RDA) for genomic comparison.
Main Results:
- Strain S1 was identified as closely related to Lactobacillus sobrius.
- Phylogenetic analysis showed high homology (99.6%) between strain S1 and L. sobrius.
- Representational difference analysis revealed no genomic differences between strain S1 and L. sobrius 001T.
Conclusions:
- Piglet-derived Lactobacillus sp. strain S1 is confirmed as Lactobacillus sobrius.
- Lactobacillus sobrius strains 001T and S1 are considered the same strain.
