Related Experiment Video
Updated: Jul 3, 2026
Isolation, Characterization, and Total DNA Extraction to Identify Endophytic Fungi in Mycoheterotrophic Plants
Published on: May 5, 2023
[Isolation and identification of endophytic fungi from Ligusticum]
Yang-Li Wang1, Zhu-Yun Yan, Xiao-Heng Guo
1Chinese Traditional Medicine Standerdization Lab, Chengdu University of traditional Chinese medicine, Chengdu 610075, China.
Objective:
To investigate the correlation between entophytic fungal community, habitations and varieties of Ligusticum.
Method:
The solidified potato dextrose agar (PDA) of plates was applied for the isolation of the endophytic fungi, and the identification was completed by spot-planting method.
Result:
Fifty strains of the entophytic fungi were isolated from the rhizome of L. chuanxiong collected from 6 habitations. They were morphologically identified as belonging to 13 genera, 4 families, 3 orders and 1 class.
Conclusion:
There were some differences at quantity, species and distributing of the entophytic fungi in different habitats and varieties of Ligusticum, which was suggested that entophytic fungal community is related with specific habitat.
