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Related Concept Videos

Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.
Serum Laboratory Studies, Stool Test, Breath Test01:30

Serum Laboratory Studies, Stool Test, Breath Test

Gastrointestinal (GI) diagnostic studies are pivotal in confirming, ruling out, diagnosing, or staging various diseases, including cancers. Following diagnosis, allocating time for discussions with the patient and providing informational resources is crucial. Diagnostic assessments of the GI tract often occur in outpatient settings like endoscopy suites or GI labs. Preparation for these tests may include dietary restrictions, fasting, liquid bowel preparations, laxatives, enemas, and the...

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Related Experiment Video

Updated: Jul 3, 2026

Development and Validation of a Quantitative PCR Method for Equid Herpesvirus-2 Diagnostics in Respiratory Fluids
09:57

Development and Validation of a Quantitative PCR Method for Equid Herpesvirus-2 Diagnostics in Respiratory Fluids

Published on: March 17, 2016

Diagnostic equine serology.

Kurt L Zimmerman1, Mark V Crisman

  • 1Department of Biomedical Sciences and Pathobiology, Virginia Maryland Regional College of Veterinary Medicine, Virginia Tech, Blacksburg, VA 24061, USA. kzimmerm@vt.edu

The Veterinary Clinics of North America. Equine Practice
|July 26, 2008
PubMed
Summary
This summary is machine-generated.

This guide helps equine clinicians identify serum tests for diagnosing equine diseases. It details sample types, assay principles, and lab resources for accurate diagnosis.

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Area of Science:

  • Veterinary Medicine
  • Immunology
  • Clinical Pathology

Background:

  • Equine clinicians require efficient diagnostic tools for various clinical presentations.
  • Accurate serologic testing is crucial for identifying infectious etiologies in horses.

Purpose of the Study:

  • To serve as a resource for equine clinicians regarding available serum tests.
  • To outline sample requirements, assay principles, and method comparisons for diagnostic assays.

Main Methods:

  • Categorization of specific etiologies by clinical diagnosis.
  • Brief commentary on disorders and their relevant diagnostic assays.
  • Abstraction of infectious disorders and summary of serologic tests.

Main Results:

  • Provides a structured overview of diagnostic assays for equine infectious diseases.
  • Details the strengths and weaknesses of various serologic testing methods.
  • Lists laboratories performing specific diagnostic assays.

Conclusions:

  • Facilitates the narrowing of differential diagnoses through serologic testing.
  • Enhances diagnostic accuracy in equine clinical practice.
  • Supports informed selection of appropriate laboratory tests for horses.