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Stabilizing Hepatocellular Phenotype Using Optimized Synthetic Surfaces

Stabilizing Hepatocellular Phenotype Using Optimized Synthetic Surfaces

Published on: September 26, 2014

Multilayer nanofilms as substrates for hepatocellular applications.

Corinne R Wittmer¹, Jennifer A Phelps, Christin M Lepus

¹Department of Chemical Engineering, Yale University, New Haven, CT 06520-8286, USA.

|July 26, 2008

Summary

Researchers explored multilayer nanofilms for liver tissue engineering. Optimal film composition, rigidity, and terminal layer are crucial for hepatic cell attachment and function, guiding biomaterial development.

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Area of Science:

Biomaterials Science
Tissue Engineering
Cell Biology

Background:

Multilayer nanofilms created via layer-by-layer (LbL) adsorption offer potential as substrates for tissue engineering applications.
Understanding cell-material interactions is crucial for developing effective tissue regeneration strategies.

Purpose of the Study:

To investigate the attachment and function of hepatic cells (HepG2, ARH, HFHb) on various multilayer nanofilms.
To identify key film properties influencing hepatic cell behavior for optimized biomaterial design.

Main Methods:

Fabrication of multilayer nanofilms using diverse polyelectrolytes (chitosan, alginate, PLL, PGA, PAH, PSS).
Evaluation of film properties including composition, terminal layer, rigidity, charge, and biofunctionalization.

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Assessment of hepatic cell attachment, confluence, and function on candidate films, with and without chemical cross-linking.

Main Results:

HepG2 cells achieved confluence on (PAH-PSS)n and cross-linked (PLL-ALG)n-PLL films.
Cross-linked PLL-ALG and PLL-PGA films with collagen supported adult rat hepatocyte (ARH) attachment and function.
Human fetal hepatoblasts (HFHb) exhibited attachment, confluence, and function on (PAH-PSS)n, cross-linked (PLL-ALG)n, and cross-linked (PLL-PGA)n-PLL films, with the latter showing the highest function.

Conclusions:

Multilayer film composition, terminal layer, and rigidity are critical factors for hepatic cell attachment and function.
While film charge and biofunctionality play a role, they are less influential than the primary factors.
These findings identify optimal multilayer biomaterials for advancing human liver tissue engineering.