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Updated: Jul 3, 2026

Isolation of Endothelial Progenitor Cells from Human Umbilical Cord Blood

Isolation of Endothelial Progenitor Cells from Human Umbilical Cord Blood

Published on: September 14, 2017

EULAR Scleroderma Trials and Research group statement and recommendations on endothelial precursor cells.

J H W Distler¹, Y Allanore, J Avouac

¹Department for Internal Medicine 3 and Institute for Clinical Immunology, University of Erlangen-Nuremberg, Erlangen, Germany.

Annals of the Rheumatic Diseases

|July 26, 2008

Summary

Standardizing research on endothelial precursor cells (EPCs) in systemic sclerosis (SSc) is crucial for understanding vascular disease. This expert panel provides recommendations to unify EPC research methods for better comparison of study results.

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Area of Science:

Rheumatology
Vascular Biology
Cell Biology

Background:

Systemic sclerosis (SSc) involves progressive microangiopathy, with endothelial precursor cells (EPCs) potentially playing a role in vascular pathogenesis.
Current research on EPCs in SSc is complicated by diverse isolation, culture, and quantification protocols, hindering result interpretation.
Standardization is needed to enable reliable comparisons across studies investigating EPCs in SSc.

Framework:

The European League Against Rheumatism Scleroderma Trials and Research (EUSTAR) expert panel convened to establish recommendations for EPC research standardization.
Consensus statements were developed focusing on methods for EPC isolation, enrichment, culture, and quantification.
Key recommendations address the detailed reporting of cardiovascular risk factors and medications.

Implementation:

For in vitro EPC culture, coating with laminin and type IV collagen is suggested to mimic in vivo conditions.
Confirmation of the endothelial phenotype is recommended for all in vitro cultures.
Specific markers (CD133, VEGFR2, CD34) combined with a viability marker are recommended for blood EPC quantification.

Implications:

Adherence to these EUSTAR recommendations will unify EPC research methodologies.
Standardized protocols will facilitate more accurate comparisons of findings across different studies.
This standardization aims to advance the understanding of vascular pathogenesis in SSc and improve patient outcomes.