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Updated: Jul 3, 2026

Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography
Published on: September 2, 2020
An integrated approach for automating validation of extracted ion chromatographic peaks
William D Nelson1, Kert Viele, Bert C Lynn
1Department of Biology, University of Kentucky, Lexington, KY 40506, USA. wnels2@uky.edu
Summary:
Accurate determination of extracted ion chromatographic peak areas in isotope-labeled quantitative proteomics is difficult to automate. Manual validation of identified peaks is typically required. We have integrated a peak confidence scoring algorithm into existing tools which are compatible with analysis pipelines based on the standards from the Institute for Systems Biology. This algorithm automatically excludes incorrectly identified peaks, improving the accuracy of the final protein expression ratio calculation.
Source And Supplementary Information:
http://www.chem.uky.edu/research/lynn/Nelson.pdf.
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