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Human serum pyroglutamyl-beta-naphthylamide hydrolyzing activity during development and aging.

J M Martínez1, M J Ramírez, I Prieto

  • 1Area de Fisiologia, Universidad de Jaén, Edif. 5, Paraje Las Lagunillas, 23071, Jaén, Spain.

Archives of Gerontology and Geriatrics
|July 29, 2008
PubMed
Summary

Pyroglutamyl-beta-naphthylamide hydrolyzing activity (pGluHA) shows distinct age-related changes and sex differences in human serum. Activity profiles varied significantly between males and females throughout development and aging.

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Area of Science:

  • Biochemistry
  • Human Physiology
  • Enzymology

Background:

  • Pyroglutamyl-beta-naphthylamide hydrolyzing activity (pGluHA) removes pyroglutamic acid from peptides like TRH and GnRH.
  • The specific physiological role of pGluHA in human serum remains largely uncharacterized.

Purpose of the Study:

  • To investigate the activity of pGluHA in human serum across different developmental and aging stages.
  • To identify potential sex-specific differences and age-related trends in pGluHA activity.

Main Methods:

  • Analysis of pGluHA activity in serum samples from 139 men and 148 women.
  • Quantification of pGluHA using the artificial substrate pGlu-beta-naphthylamide.

Main Results:

  • Observed significant sex differences and age-related changes in pGluHA activity in both men and women.
  • Men showed increased activity until puberty, with no substantial post-pubertal changes. Women exhibited increased activity in older age groups.
  • A significant positive correlation between pGluHA activity and age was found in the total population and within each sex.

Conclusions:

  • pGluHA activity in human serum exhibits distinct developmental and aging patterns that differ between sexes.
  • These findings suggest potential alterations in the functional status of circulating substrates influenced by age and sex.
  • Further research is warranted to elucidate the precise physiological implications of these observed pGluHA activity changes.