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Published on: April 11, 2016
Common approaches for efficient method development with immobilised polysaccharide-derived chiral stationary phases
1Chiral Technologies Europe, Parc d'Innovation, Bd. Gonthier d'Andernach, B.P. 80140, F-67404 Illkirch Cedex, France. pfranco@chiral.fr
Summary
This study introduces novel immobilised polysaccharide-based chiral stationary phases (CSPs) for efficient enantiomeric resolution. Method development strategies using limited mobile phases in liquid and supercritical chromatography (LC and SFC) achieve high success rates.
Area of Science:
- Chromatography
- Chiral Separations
- Analytical Chemistry
Background:
- Immobilised polysaccharide-based chiral stationary phases (CSPs) represent advanced chromatographic materials.
- These CSPs offer excellent enantioselective performance and solvent versatility for separating enantiomers.
Purpose of the Study:
- To present an efficient method development approach using immobilised CSPs (CHIRALPAK IA, IB, IC).
- To review screening strategies for enantiomeric separation via liquid chromatography (LC) and supercritical fluid chromatography (SFC).
Main Methods:
- Utilisation of a limited set of mobile phases for method development.
- Application of screening strategies in both LC and SFC modes.
- Focus on CHIRALPAK IA, CHIRALPAK IB, and CHIRALPAK IC immobilised CSPs.
Main Results:
- Demonstration of efficient enantiomeric resolution using the developed strategies.
- Successful application of combined LC and SFC modes for comprehensive screening.
- Achieved high success rates in obtaining enantiomeric separations.
Conclusions:
- The rational combination of LC and SFC with immobilised CSPs enables efficient enantiomeric resolution.
- This approach allows for obtaining resolutions within reasonable time frames.
- The developed strategies provide a high success rate for chiral separations.
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