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Updated: Jul 3, 2026
Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules
Published on: November 2, 2009
The study of oxidization fluorescence sensor with molecular imprinting polymer and its application for
1Department of Chemistry, School of Chemistry and Materials Science, Shaanxi Normal University, Xi'an 710062, PR China.
Abstract:
This paper developed optical fiber sensor based on molecular imprinted polymer as artificial recognition element for the determination of 6-mercaptopurine (6-MP) in human serum. This approach displayed high sensitivity by oxidizing 6-MP to a strong fluorescent compound with H(2)O(2) in the alkaline media. It offered a relatively nice selectivity for 6-MP detection by molecular imprinted polymer's recognition. The relative standard deviation (R.S.D.) was 5% for a same sensor (n=5) when 6-MP concentration was 1.0 x 10(-7)gm L(-1) in serum. The developed method was satisfactorily applied to the determination of 6-MP in human serum without any necessity for sample treatment or time-consuming extraction steps prior to the analysis.
