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Updated: Jul 3, 2026

A Fluorescence-based Method to Study Bacterial Gene Regulation in Infected Tissues

A Fluorescence-based Method to Study Bacterial Gene Regulation in Infected Tissues

Published on: February 19, 2019

Mycobacterium tuberculosis sigma factor E regulon modulates the host inflammatory response.

Patricia A Fontán¹, Virginie Aris, María E Alvarez

¹Public Health Research Institute, International Center for Public Health, New Jersey Medical School, University of Medicine and Dentistry of New Jersey, Newark, NJ 07103-3535, USA.

The Journal of Infectious Diseases

|July 29, 2008

Summary

Mycobacterium tuberculosis uses Sigma factor E (SigE) to maintain cell envelope integrity during macrophage infection. This SigE regulon also influences the host

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Area of Science:

Microbiology
Immunology
Molecular Biology

Background:

Mycobacterium tuberculosis (Mtb) persists within macrophages, evading host defenses.
Understanding host-pathogen interactions is crucial for developing novel tuberculosis treatments.
Gene regulation is key to Mtb's intracellular survival and virulence.

Purpose of the Study:

To identify the SigE regulon in Mtb during macrophage infection.
To investigate the impact of the SigE regulon on Mtb's transcriptional response.
To analyze the effect of the SigE regulon on the host phagocyte's transcriptional response.

Main Methods:

Macrophage infection models with Mtb.
Transcriptional analysis of Mtb genes regulated by SigE.
Analysis of host cell transcriptional changes in response to Mtb infection.

Main Results:

The SigE regulon was identified during Mtb macrophage infection.
SigE regulates genes critical for Mtb cell envelope integrity and function within macrophages.
The SigE regulon modulates the host's inflammatory transcriptional response.

Conclusions:

SigE is essential for Mtb adaptation and survival within macrophages.
The SigE regulon plays a dual role in bacterial virulence and host immune modulation.
Targeting the SigE pathway could offer new therapeutic strategies against tuberculosis.