Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Solid-phase gene assembly.

K L Beattie1, R F Fowler

  • 1Center for Biotechnology, Baylor College of Medicine, The Woodlands, Texas 77381.

Nature
|August 8, 1991
PubMed
Summary
This summary is machine-generated.

New chemical synthesis and DNA hybridization technologies enable cost-effective, rapid production of long DNA regions. These advancements may revolutionize protein engineering, opening new possibilities for research and development.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

DNA microarrays detect 4-nonylphenol-induced alterations in gene expression during zebrafish early development.

Ecotoxicology (London, England)·2003
Same author

Towards environmental toxicogenomics -- development of a flow-through, high-density DNA hybridization array and its application to ecotoxicity assessment.

The Science of the total environment·2001
Same author

Analysis of nucleic acids by tandem hybridization on oligonucleotide microarrays.

Methods in molecular biology (Clifton, N.J.)·2001
Same author

Screening for ribosomal-based false positives following prokaryotic mRNA differential display.

BioTechniques·2001
Same author

Modification of an automated liquid-handling system for reagent-jet, nanoliter-level dispensing.

BioTechniques·2001
Same author

Pyrophosphorolytic dismutation of oligodeoxy-nucleotides by terminal deoxynucleotidyltransferase.

Nucleic acids research·1999
Same journal

Incoming US science academy chief vows to 'double down' on research.

Nature·2026
Same journal

Author Correction: Synthesis of enantioenriched atropisomers by biocatalytic deracemization.

Nature·2026
Same journal

Electrodeposited self-assembled molecules for perovskite photovoltaics.

Nature·2026
Same journal

Neutrino's nursery found: the 'Shadow Blaster'.

Nature·2026
Same journal

Dementia risk in middle-aged people linked to a blood protein.

Nature·2026
Same journal

Daily briefing: What's really happening with trust in science.

Nature·2026
See all related articles

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Synthetic Chemistry

Background:

  • Traditional methods for synthesizing long DNA sequences are often slow and expensive.
  • The demand for longer DNA constructs is increasing in various fields, including protein engineering.

Purpose of the Study:

  • To evaluate the impact of new oligonucleotide synthesis and hybridization technologies on DNA preparation.
  • To explore the potential of these advancements for future applications in protein engineering.

Main Methods:

  • Utilized novel chemical synthesis techniques for oligonucleotides.
  • Employed stepwise hybridization on a solid-phase support for DNA assembly.

Main Results:

  • Achieved rapid and cost-effective preparation of long duplex DNA regions.

Related Experiment Videos

  • Demonstrated the efficiency of the new solid-phase hybridization approach.

    • Conclusions:

    • The developed technologies offer a significant improvement in DNA synthesis and assembly.
    • These advancements are poised to accelerate innovation in protein engineering and related biotechnologies.