Related Experiment Video
Updated: Jul 3, 2026
Culturing and Measuring Fetal and Newborn Murine Long Bones
Published on: April 26, 2019
[Study of nHACP/CF co-cultured with osteoblasts in vitro]
Ning-Ning Sun1, Xu-Kai Wang, Qing Zhou
1Department of Oral and Maxillofacial Surgery, School of Stomatology,China Medical University. Shenyang 110002, Liaoning Province, China. clare_ning@yahoo.com.cn
Purpose:
To investigate the effect of nHACP/CF on coherence and proliferation of osteoblast.
Methods:
The rat osteoblasts were isolated and cultured, then co-cultured with nHACP/CF in vitro. The cells were observed under cytochemical staining for ALP; the cell-material complex was observed under electronic scanning microscope, MTT was used for detection of cell proliferation,and ALP activity was determined at different times.SPSS 11.5 software package was used for one-way ANOVA.
Results:
Cytochemical staining for ALP was positive. The best density of seeding cells was 6x10(5)/ml; osteoblast could attach and extend on nHACP/CF,the proliferation and growth were not affected by nHACP/CF.
Conclusion:
nHACP/CF is a good vehicle for rat osteoblast to grow on.
