Related Experiment Video
Updated: Jul 3, 2026

T4 Bacteriophage and E. coli Interaction in the Murine Intestine: A Prototypical Model for Studying Host-Bacteriophage Dynamics In Vivo
Published on: January 26, 2024
Partial characterization of Enterococcus faecalis bacteriophage F4
K Nigutová1, I Styriak, P Javorský
1Institute of Animal Physiology, Slovak Academy of Sciences, Kosice, Slovakia. nigutova@saske.sk
Abstract:
Large Enterococcus faecalis F4 bacteriophage (described earlier) consisting of double-stranded linear DNA of approximately 60 kb was characterized. Library was prepared of its random DNA fragments and selected recombinants were sequenced. Three phage essential genes were characterized: DNA polymerase, replicative DNA helicase and a minor capsid protein, showing only limited homology to other known phage encoded genes. The occurrence of these genes among enterococci was determined by PCR method. Only two out of 40 tested isolates possessed all three genes, another three isolates contained at least one of the genes, demonstrating low frequency F4 lysogens among natural enterococcal isolates.
Related Concept Videos
DNA Bacteriophages
Viral Replication: Lytic Cycle
Lytic Cycle of Bacteriophages
Bacteriophages of the Human Virome
Lysogenic Cycle of Bacteriophages
Conjugation

