Related Experiment Video
Updated: Jul 3, 2026

Selective Cleaning of Wild Caenorhabditis Nematodes to Enrich for Intestinal Microbiome Bacteria
Published on: August 13, 2021
Distinctive archaebacterial species associated with anaerobic rumen protozoan Entodinium caudatum
T Tóthová1, M Piknová, S Kisidayová
1Department of Biochemistry, Institute of Chemistry, P.J. Safárik University, 040 01 Kosice, Slovakia.
Abstract:
The diversity of archaebacteria associated with anaerobic rumen protozoan Entodinium caudatum in long term in vitro culture was investigated by denaturing gradient gel electrophoresis (DGGE) analysis of hypervariable V3 region of archaebacterial 16S rRNA gene. PCR was accomplished directly from DNA extracted from a single protozoal cell and from total community genomic DNA and the obtained fingerprints were compared. The analysis indicated the presence of a solitary intensive band present in Entodinium caudatum single cell DNA, which had no counterparts in the profile from total DNA. The identity of archaebacterium represented by this band was determined by sequence analysis which showed that the sequence fell to the cluster of ciliate symbiotic methanogens identified recently by 16S gene library approach.
Related Concept Videos
Diversity of Archaea II
Diversity of Archaea III
Diversity of Protists I
Diversity of Archaea I
Microbiota of the Large Intestine
Diversity of Protists II

