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[Study of Tripterygium hypoglaucum Hutch on mouse's splenic lymphocyte activation in vitro]
Yan Xu1, Yong-Tang Zheng, Li He
1Department of Dermatology, First Affiliated Hospital of Kunming College, Kunming 650032, China. xuyanfirst@163.com
Objective:
To study the effects of Tripterygium hypoglaucum Hutch (THH) on the mouse's splenic lymphocyte proliferation, activation mark such as CD69 and CD25, cytokine such as IL-4 and gamma-IFN.
Methods:
Separating the mouse's splenic lymphocyte sterilitily and adding THH of different density. The proliferation of the ConA-stimulated splenic lymphocyte was measured with MTT; the activation marks such as CD69 and CD25 were measured with flow cytometer; cytokines such as IL-4 and gamma-IFN were measured with euzymelinked immunosorbent assay.
Results:
THH had inhibitory action on both proliferation and activation of splenic lymphocyte.
