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LPS and ATP-induced Death of PMA-differentiated THP-1 Macrophages and its Validation
Published on: May 3, 2024
Oxidative lipidomics of programmed cell death.
Vladimir A Tyurin1, Yulia Y Tyurina, Patrick M Kochanek
1Center for Free Radical and Antioxidant Health, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.
Methods in Enzymology
|July 30, 2008
Summary
Oxidative lipidomics, a new method, quantifies oxidized phospholipids like cardiolipin and phosphatidylserine during apoptosis. This technique reveals their role in cell death and inflammation across various tissues and conditions.
Area of Science:
- Biochemistry
- Cell Biology
- Analytical Chemistry
Background:
- Oxidized phospholipids are crucial in apoptosis execution and the clearance of apoptotic cells by macrophages.
- Accurate identification and quantification of these oxidized phospholipids are essential for understanding cellular processes.
Purpose of the Study:
- To introduce and validate oxidative lipidomics as a method for identifying and quantifying oxidized phospholipids.
- To characterize the selective oxidation of cardiolipin (CL) and phosphatidylserine (PS) during apoptosis.
Main Methods:
- Utilized a novel oxidative lipidomics approach combining electrospray ionization/mass spectrometry (ESI-MS) and fluorescence high-performance liquid chromatography.
- Analyzed oxidized molecular species of CL and PS in various in vitro and in vivo models.
Main Results:
- Demonstrated the accumulation of specific oxidized cardiolipin species during apoptosis.
- Identified oxidized phosphatidylserine with oxidized C(22:6) as a major oxidized species in multiple models, including traumatic brain injury, Alzheimer's disease postmortem samples, and gamma-irradiated mouse intestine.
- Showcased the presence of CL hydroperoxides in traumatic brain injury models.
Conclusions:
- Oxidative lipidomics is a powerful technique for studying lipid oxidation in cell death and inflammation.
- This method enables the quantification of specific oxidized phospholipids and their hydroperoxides.
- The findings highlight the significance of oxidized CL and PS in various pathological conditions.
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