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Published on: May 10, 2014
Molecular cloning, expression and functional characterization of the chicken cation dependent mannose 6-phosphate
Praveen Kumar Amancha1, Siva Kumar Nadimpalli
1Protein Biochemistry and Molecular Biology Laboratory, Department of Biochemistry, University of Hyderabad, Hyderabad 500 046, India.
Abstract:
Mammalian mannose 6-phosphate (M6P) receptors function in transport of lysosomal enzymes. To understand the structural and functional significance of the chicken cation dependent mannose 6-phosphate receptor (MPR) (Mr 46 kDa), a full-length cDNA for the chicken protein was cloned and expressed in mpr(-/-) MEF cells devoid of both the receptors. The stably transfected cells express the receptor that could be affinity purified by phosphomannan chromatography. The authenticity of the receptor was confirmed by its immuno-reactivity with mammalian MPR 46 antibodies and its ability to sort cathepsin D in transfected cells (92.3%) as compared to mock transfected cells (50.2%), establishing a functional role for the chicken receptor.
