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Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
Next-generation Sequencing03:00

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.
Sanger Sequencing01:57

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
DNA as a Genetic Template02:05

DNA as a Genetic Template

Two structural features of the DNA molecule provide a basis for the mechanisms of heredity: the four nucleotide bases and its double-stranded nature. The Watson-Crick model of double-helical DNA structure, proposed in 1952, drew heavily upon the X-ray crystallography work of researchers Rosalind Franklin and Maurice Wilkins. Watson, Crick, and Wilkins jointly received the Nobel Prize in Physiology or Medicine for their work in 1962. Franklin was, controversially, excluded from the prize for...
DNA as a Genetic Template02:05

DNA as a Genetic Template

Two structural features of the DNA molecule provide a basis for the mechanisms of heredity: the four nucleotide bases and its double-stranded nature. The Watson-Crick model of double-helical DNA structure, proposed in 1952, drew heavily upon the X-ray crystallography work of researchers Rosalind Franklin and Maurice Wilkins. Watson, Crick, and Wilkins jointly received the Nobel Prize in Physiology or Medicine for their work in 1962. Franklin was, controversially, excluded from the prize for...

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Updated: Jul 3, 2026

High-Density DNA and RNA microarrays - Photolithographic Synthesis, Hybridization and Preparation of Large Nucleic Acid Libraries
11:22

High-Density DNA and RNA microarrays - Photolithographic Synthesis, Hybridization and Preparation of Large Nucleic Acid Libraries

Published on: August 12, 2019

Do DNA microarrays have their future behind them?

Jean-Yves Coppée1

  • 1DNA microarray platform, Institut Pasteur, 75015 Paris, France. jycoppee@pasteur.fr

Microbes and Infection
|July 30, 2008
PubMed
Summary
This summary is machine-generated.

DNA microarray technology has evolved significantly since 1995, expanding from gene expression to diverse applications including clinical diagnostics. Despite facing competition from sequencing, its utility remains relevant, presenting a paradoxical situation.

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DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning
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Last Updated: Jul 3, 2026

High-Density DNA and RNA microarrays - Photolithographic Synthesis, Hybridization and Preparation of Large Nucleic Acid Libraries
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Published on: August 12, 2019

DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning
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ampliPHOX Colorimetric Detection on a DNA Microarray for Influenza
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Area of Science:

  • Molecular Biology
  • Genomics
  • Biotechnology

Background:

  • DNA microarray technology, first reported in 1995, has evolved significantly beyond its initial application in gene expression profiling.
  • Its utility has expanded across various scientific fields, including microbiology and cancer research.
  • The technology is now integral for detecting single nucleotide polymorphisms, microRNAs, copy number variations, and CpG methylations.

Purpose of the Study:

  • To review the evolution and diverse applications of DNA microarray technology.
  • To discuss the current paradoxical situation where DNA arrays face competition from next-generation sequencing (NGS) technologies.
  • To explore the ongoing relevance and future prospects of DNA microarrays in diagnostics and research.

Main Methods:

  • Literature review of DNA microarray technology advancements and applications.
  • Analysis of the impact of next-generation sequencing on microarray usage.
  • Discussion of regulatory approvals and clinical diagnostic applications.

Main Results:

  • DNA microarrays are routinely employed for various genetic analyses and have gained FDA approval for clinical diagnostics.
  • Despite the rise of high-throughput sequencing, microarrays continue to offer unique advantages in specific applications.
  • The field faces a dynamic landscape with emerging technologies challenging established methods.

Conclusions:

  • DNA microarray technology demonstrates remarkable adaptability and continued relevance in molecular biology and diagnostics.
  • The coexistence of microarrays and sequencing technologies highlights a complex, evolving technological ecosystem.
  • Further research and development are crucial to define the future role of microarrays amidst rapid technological advancements.