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In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

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Related Experiment Video

Updated: Jul 3, 2026

Mouse Sperm Cryopreservation and Recovery using the I&middot;Cryo Kit
07:06

Mouse Sperm Cryopreservation and Recovery using the I·Cryo Kit

Published on: December 12, 2011

Conserving, distributing and managing genetically modified mouse lines by sperm cryopreservation.

G Charles Ostermeier1, Michael V Wiles, Jane S Farley

  • 1Technology Evaluation and Development, The Jackson Laboratory, Bar Harbor, Maine, United States of America.

Plos One
|July 31, 2008
PubMed
Summary

Cryopreservation of C57BL/6 mouse sperm is now efficient. A new method improves fertilization rates for genetically modified mouse sperm archiving and distribution.

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Using a Comparative Species Approach to Investigate the Neurobiology of Paternal Responses

Published on: September 19, 2011

Related Experiment Videos

Last Updated: Jul 3, 2026

Mouse Sperm Cryopreservation and Recovery using the I&middot;Cryo Kit
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Published on: December 12, 2011

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Using a Comparative Species Approach to Investigate the Neurobiology of Paternal Responses
07:59

Using a Comparative Species Approach to Investigate the Neurobiology of Paternal Responses

Published on: September 19, 2011

Area of Science:

  • Reproductive Biology
  • Cryobiology
  • Genetics

Background:

  • Sperm from C57BL/6 mice present challenges for cryopreservation and recovery.
  • The C57BL/6 genetic background is widely used for genetically modified (GM) mouse lines.

Purpose of the Study:

  • To develop an efficient and reliable method for cryopreserving and recovering sperm from C57BL/6 mice.
  • To assess the broad applicability of this method for archiving and distributing GM mouse lines.

Main Methods:

  • Sperm collected from vas deferens and epididymis.
  • Cryoprotective medium: 18% raffinose, 3% skim milk, 477 μM monothioglycerol.
  • Controlled cooling (37°C/min) and warming (2,232°C/min) rates, storage in liquid nitrogen.

Main Results:

  • Achieved fertilization rates of 70±5% for C57BL/6 sperm, a six-fold increase.
  • Successfully cryopreserved and recovered sperm from 735 GM mouse lines across 12 genetic backgrounds.
  • Equivalent or superior embryo developmental capacity compared to cryopreserved in vitro fertilization (IVF)-derived embryos.

Conclusions:

  • The developed method is broadly applicable for archiving and distributing mice.
  • This approach offers an economical and efficient solution for maintaining GM mouse lines.
  • Cryopreservation of sperm from various mouse strains, including C57BL/6, is now feasible with high success rates.