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Related Concept Videos

Modern Molecular Taxonomy01:29

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Related Experiment Video

Updated: Jul 3, 2026

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
11:22

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing

Published on: October 15, 2019

Comparative analysis of human gut microbiota by barcoded pyrosequencing.

Anders F Andersson1, Mathilda Lindberg, Hedvig Jakobsson

  • 1Swedish Institute for Infectious Disease Control, Solna, Sweden.

Plos One
|July 31, 2008
PubMed
Summary

This study introduces a high-throughput sequencing method for analyzing microbial communities. The technique accurately characterizes microbial composition, aiding in the discovery of therapeutic targets for diseases linked to microbial imbalance.

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Last Updated: Jul 3, 2026

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
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Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing
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Published on: March 19, 2018

Identification of Metabolically Active Bacteria in the Gut of the Generalist Spodoptera littoralis via DNA Stable Isotope Probing Using 13C-Glucose
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Area of Science:

  • Microbial Ecology
  • Genomics
  • Bioinformatics

Background:

  • Human health is influenced by complex microbial communities.
  • Microbial imbalances are linked to disease development.
  • Accurate microbiota characterization is crucial for identifying therapeutic targets.

Purpose of the Study:

  • To develop a high-throughput, cost-effective method for microbiota characterization.
  • To enable parallel, in-depth analysis of numerous samples.
  • To apply the method for analyzing microbial communities in various human body sites.

Main Methods:

  • Utilized 454-pyrosequencing of the 16S rRNA gene's hyper-variable region.
  • Incorporated sample-specific barcode sequences for parallel analysis.
  • Performed in silico modeling to validate the method's accuracy down to phylotype level.

Main Results:

  • Demonstrated the method's ability to accurately describe microbial communities.
  • Successfully applied barcoded pyrosequencing to analyze throat, stomach, and fecal samples.
  • Confirmed the technique's suitability for high-throughput comparative microbial ecology.

Conclusions:

  • Barcoded pyrosequencing is a powerful tool for microbial ecology studies.
  • This method facilitates in-depth analysis of microbial communities across different body sites.
  • The technique supports the identification of microbial signatures associated with health and disease.