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Updated: Jul 3, 2026
Creation of Colonic Anastomosis in Mice
Published on: January 17, 2019
Colonic perianastomotic carcinogenesis in an experimental model
Sergio Pérez-Holanda1, Luis Rodrigo, Carme Pinyol-Felis
1Department of Surgery, Hospital Valle Del Nalón, Langreo, Asturias, Spain. sergio.perez-holanda@sespa.princast.es
Background:
To examine the effect of anastomosis on experimental carcinogenesis in the colon of rats.
Methods:
Forty-three 10-week-old male and female Sprague-Dawley rats were operated on by performing an end-to-side ileorectostomy. Group A:16 rats received no treatment. Group B: 27 rats received 18 subcutaneous injections weekly at a dose of 21 mg/kg wt of 1-2 dimethylhydrazine (DMH), from the eighth day after the intervention. Animals were sacrificed between 25-27 weeks. The number of tumours, their localization, size and microscopic characteristics were recorded. A paired chi-squared analysis was performed comparing tumoral induction in the perianastomotic zone with the rest of colon with faeces.
Results:
No tumours appeared in the dimethylhydrazine-free group. The percentage tumoral area was greater in the perianastomotic zone compared to tumours which had developed in the rest of colon with faeces (p = 0.014).
Conclusion:
We found a cocarcinogenic effect due to the creation of an anastomosis, when using an experimental model of colonic carcinogenesis induced by DMH in rats.
