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Published on: August 6, 2014
Fluorescence generation from tandem repeats of a malachite green RNA aptamer using rolling circle transcription
Kazuhiro Furukawa1, Hiroshi Abe, Naoko Abe
1Nano Medical Engineering Laboratory, Advanced Science Institute, RIKEN 2-1, Hirosawa, Wako-Shi, Saitama 351-0198, Japan.
Abstract:
We demonstrate a generation of tandem repeats of a malachite green (MG) RNA aptamer using rolling circle transcription. To keep the higher-order structure of each aptamer on long RNA, we designed a sequence of circular DNA with a 14-base linker. T7 RNA polymerase was superior to Escherichia coli RNA polymerase in the specific transcription of the MG RNA aptamer. Finally, the generation of the fluorescence signal was confirmed from aptamer repeats with MG.

