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Published on: May 1, 2020
[Effeet of rapamycin on mTOR and eIF-4E expression in coxsackievirus B3-induced rat myocardial cells]
Chun-Yuan Chen1, Yue-Nu Sun, Zuo-Cheng Yang
1Department of Pediatics, Third Xiangya Hospital, Central South University, Changsha 410013, China.
Objective:
To observe the effeet of rapamycin, an inhibitor of mammalian target of rapamycin (mTOR), on mTOR and eukaryotic initiation factor-4E(eIF-4E)expression in coxsac-kievirus B3 (CVB3)-induced rat myocardial cells and to investigate the role of mTOR/eIF-4E signal pathway in viral myocarditis.
Methods:
To construct a cell model of viral myocarditis with primary cultured myocardial cells. Myocardial cells infected by CVB3 were treated with 10 nmol/L rapamycin according to the cell toxicity test. The mTOR and eIF-4E expressions of cells were determined by RT-PCR and Western Blot.
Results:
Rapamycin inhibited the degeneration of CVB3-induced myocardial cells. Expressions of mTOR and eIF-4E mRNA or protein in CVB3-induced myocardial cells were significantly upregulated compared with the control group (P < 0.05), and rapamycin (10 nmol/L) inhibited the upregulation (P < 0.05).
Conclusion:
Rapamycin can downregulate the expressions of mTOR and eIF-4E in CVB3-induced myocardial cells, suggesting that mTOR/eIF-4E signal transduction may play an important role in viral myocarditis.
Insights
Rapamycin treatment reduced mammalian target of rapamycin (mTOR) and eukaryotic initiation factor-4E (eIF-4E) expression in coxsackievirus B3-induced myocarditis. This suggests the mTOR/eIF-4E pathway is crucial in viral myocarditis.
Area of Science:
- Cardiology
- Virology
- Molecular Biology
Background:
- Viral myocarditis is a serious cardiac condition.
- The mammalian target of rapamycin (mTOR)/eukaryotic initiation factor-4E (eIF-4E) signaling pathway is implicated in cellular processes.
- Understanding molecular pathways in viral myocarditis is critical for developing treatments.
Purpose of the Study:
- To investigate the effect of rapamycin on mTOR and eIF-4E expression in coxsackievirus B3 (CVB3)-induced rat myocardial cells.
- To explore the role of the mTOR/eIF-4E signaling pathway in the pathogenesis of viral myocarditis.
Main Methods:
- Primary cultured myocardial cells were used to create a viral myocarditis cell model.
- Cells were infected with CVB3 and treated with rapamycin (10 nmol/L).
- Gene and protein expression levels of mTOR and eIF-4E were analyzed using RT-PCR and Western Blot.
Main Results:
- Rapamycin treatment mitigated the degeneration of CVB3-induced myocardial cells.
- Expressions of mTOR and eIF-4E mRNA and protein were significantly upregulated in CVB3-infected cells compared to controls (P < 0.05).
- Rapamycin treatment significantly inhibited this upregulation (P < 0.05).
Conclusions:
- Rapamycin effectively downregulates mTOR and eIF-4E expression in CVB3-induced myocardial cells.
- The mTOR/eIF-4E signaling pathway plays a significant role in the development of viral myocarditis.
- These findings suggest potential therapeutic targets for viral myocarditis.
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