Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Induced Pluripotent Stem Cells

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic cells are...

Induced Pluripotent Stem Cells

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore called induced pluripotent stem...

Induced Pluripotent Stem Cells

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore called induced pluripotent stem...

Stem Cell Culture

Stem Cell Culture

Stem cell research aims to find ways to use stem cells to regenerate and repair cellular damage. Over time, most adult cells undergo the wear and tear of aging and lose their ability to divide and repair themselves. Stem cells do not display a particular morphology or function. Adult stem cells, which exist as a small subset of cells in most tissues, keep dividing and can differentiate into a number of specialized cells generally formed by that tissue. These cells enable the body to renew and...

Somatic to iPS Cell Reprogramming

Somatic to iPS Cell Reprogramming

Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012 for this...

Embryonic Stem Cells

Embryonic Stem Cells

Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Delphi-driven consensus definition for mesenchymal stromal cells and clinical reporting guidelines for mesenchymal stromal cell-based therapeutics.

Cytotherapy·2024

Same author

Challenges and Considerations of Preclinical Development for iPSC-Based Myogenic Cell Therapy.

Cells·2024

Same author

Making NSC and Neurons from Patient-Derived Tissue Samples.

Methods in molecular biology (Clifton, N.J.)·2019

Same author

Special issue on stem cell and tissue engineering in development, disease, and repair.

Developmental dynamics : an official publication of the American Association of Anatomists·2018

Same author

Exome sequencing in families with severe mental illness identifies novel and rare variants in genes implicated in Mendelian neuropsychiatric syndromes.

Psychiatry and clinical neurosciences·2018

Same author

Treatment of an Ectopic Pregnancy: An Ethical Reanalysis.

The Linacre quarterly·2018

Search research articles

Related Experiment Video

Updated: Jul 3, 2026

Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions

Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions

Published on: November 27, 2017

Regulatory issues for personalized pluripotent cells.

Maureen L Condic¹, Mahendra Rao

¹Department of Neurobiology and Anatomy, University of Utah School of Medicine, Salt Lake City, Utah 84132-3401, USA. mlcondic@neuro.utah.edu

Stem Cells (Dayton, Ohio)

|August 2, 2008

Summary

This summary is machine-generated.

Personalized pluripotent stem cells offer therapeutic hope but face challenges. Induced pluripotent stem cells (iPSCs) show great promise for regenerative medicine due to fewer ethical concerns and long-term potential.

More Related Videos

Scalable 96-well Plate Based iPSC Culture and Production Using a Robotic Liquid Handling System

Scalable 96-well Plate Based iPSC Culture and Production Using a Robotic Liquid Handling System

Published on: May 14, 2015

Related Experiment Videos

Last Updated: Jul 3, 2026

Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions

Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions

Published on: November 27, 2017

Scalable 96-well Plate Based iPSC Culture and Production Using a Robotic Liquid Handling System

Scalable 96-well Plate Based iPSC Culture and Production Using a Robotic Liquid Handling System

Published on: May 14, 2015

Area of Science:

Biotechnology
Regenerative Medicine
Stem Cell Biology

Background:

Personalized pluripotent stem cells hold significant promise for both research and potential patient therapies.
Technical and regulatory hurdles must be overcome before these cells can be utilized as therapeutic agents.
Two primary sources of personalized pluripotent stem cells are nuclear transfer-derived embryonic stem cells (NT-ESCs) and induced pluripotent stem cells (iPSCs).

Purpose of the Study:

To evaluate the potential of personalized pluripotent stem cells for therapeutic applications.
To compare the regulatory and ethical challenges associated with NT-ESCs and iPSCs.
To highlight the advantages of iPSCs in the field of regenerative medicine.

Main Methods:

Review of existing literature on stem cell derivation and reprogramming.
Analysis of regulatory landscapes for different stem cell sources.
Comparative assessment of ethical considerations for NT-ESCs, iPSCs, and fertilized embryo-derived ESCs.

Main Results:

Both NT-ESCs and iPSCs face unique and significant regulatory challenges.
iPSCs present fewer ethical concerns compared to both ESCs and NT-ESCs.
Direct reprogramming to generate iPSCs is a highly promising approach for regenerative medicine.

Conclusions:

Induced pluripotent stem cells (iPSCs) offer a promising avenue for advancing regenerative medicine research and therapies.
The relative ethical advantages and long-term potential of iPSCs position them favorably over other personalized stem cell sources.
Overcoming technical and regulatory challenges is crucial for the clinical translation of personalized pluripotent stem cells.