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Verification of reference genes for relative quantification of gene expression by real-time reverse transcription PCR

Katerina Svobodová1, Karel Bílek, Ales Knoll

  • 1Department of Animal Morphology, Physiology and Genetics, Mendel University of Agriculture and Forestry, Brno, Czech Republic. kat.svobodova@centrum.cz

Journal of Applied Genetics
|August 2, 2008
PubMed

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Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...