Enhancing fluorescence detection with a photonic crystal structure in a total-internal-reflection configuration

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Fabrication And Characterization Of Photonic Crystal Slow Light Waveguides And Cavities
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Fabrication And Characterization Of Photonic Crystal Slow Light Waveguides And Cavities

Published on: November 30, 2012

Jing Yong Ye1, Mitsuru Ishikawa

  • 1Center for Ultrafast Optical Science, University of Michigan, 2200 Bonisteel Boulevard, Ann Arbor, Michigan 48109-2099, USA. jyye@umich.edu

Optics Letters
|August 2, 2008
PubMed

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Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
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