Elevated expression of L-selectin ligand in lymph node-derived human prostate cancer cells correlates with increased
Prakash Radhakrishnan1, Ming-Fong Lin, Pi-Wan Cheng
1Department of Biochemistry and Molecular Biology, College of Medicine, 985870, Nebraska Medical Center, University of Nebraska Medical Center, Omaha, NE 68198, USA.
Abstract:
Human prostate cancer LNCaP cells including C-33 and C-81 cells were originally derived from the lymph nodes of a patient with metastatic prostate cancer. These two cells were employed for characterization of L-selectin ligand and in vitro tumorigenicity, because they mimic the clinical conditions of early and late-stage human prostate cancer. C-81 cells exhibit higher in vitro migratory and invasive properties as compared with C-33 cells. We find that the L-selectin ligand and mucin glycan-associated MECA-79 epitope were elevated in C-81 cells. An increase of these glycotopes positively correlates with elevated tumorigenicity and expression of key glycosyl- and sulfotransferase genes. These results suggest that modulated expression of selective glycogenes correlates with altered tumorigenicity of cancer cells.
