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Published on: May 30, 2025
Natural infections by Cryptosporidium sp. in farm-raised ducks and geese
D Richter1, G Wiegand-Tripp, E Burkhardt
1Institut fur Geflugelkrankheiten, Justus-Liebig-Universitat Giessen, Giessen, Bundesrepublik Deutschland.
Abstract:
Modified Ziehl-Neelsen staining and an indirect immunofluorescent assay appeared to be equally useful for monitoring natural infections with Cryptosporidium sp. in farm-raised Pekin ducklings and Danish goslings. In 73 of 128 ducklings, and in 44 of 74 goslings, Cryptosporidium sp. were detected. Tissues from the bursa of Fabricius are most frequently positive in both species. The presence or absence of the parasite could not be correlated with clinical signs or lesions and/or poor performance of the birds. Oocysts from ducklings could not be transmitted to suckling mice but were infectious for ducklings. Oocysts from calves were infectious for mice but not for ducklings. These findings support the view that mammals and birds have different Cryptosporidium spp.
Insights
Modified Ziehl-Neelsen staining and immunofluorescent assays effectively detect Cryptosporidium in ducklings and goslings. Bird and mammal Cryptosporidium species appear distinct, impacting host specificity.
Area of Science:
- Veterinary Parasitology
- Avian Health
- Microbiology
Background:
- Cryptosporidium sp. infections pose a challenge in poultry farming.
- Accurate diagnostic methods are crucial for monitoring and control.
- Understanding host specificity is key to preventing disease transmission.
Purpose of the Study:
- To evaluate diagnostic techniques for Cryptosporidium in Pekin ducklings and Danish goslings.
- To determine the prevalence and tissue distribution of Cryptosporidium in these birds.
- To investigate the host specificity of avian Cryptosporidium isolates.
Main Methods:
- Modified Ziehl-Neelsen staining for parasite detection.
- Indirect immunofluorescent assay (IFA) for Cryptosporidium identification.
- Experimental transmission studies in ducklings and mice.
Main Results:
- Both Ziehl-Neelsen and IFA showed equal efficacy in detecting Cryptosporidium.
- Prevalence: 57% in ducklings (73/128) and 60% in goslings (44/74).
- Bursa of Fabricius was the most frequently infected tissue; no correlation with clinical signs.
Conclusions:
- Modified Ziehl-Neelsen and IFA are reliable for monitoring Cryptosporidium in poultry.
- Avian Cryptosporidium isolates are not infectious to mice, suggesting species-specific differences.
- Mammalian and avian Cryptosporidium likely represent distinct species, necessitating species-specific control strategies.
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