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A simple method for immunofluorescence staining of tracheal organ cultures for the rapid identification of infectious

P S Bhattacharjee1, C J Naylor, R C Jones

  • 1University of Liverpool, Department of Veterinary Pathology, Neston, South Wirral, UK.

Avian Pathology : Journal of the W.V.P.A
|September 1, 1994
PubMed

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Immunofluorescence Microscopy01:12

Immunofluorescence Microscopy

A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
The...