Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jul 3, 2026

Ex Vivo Infection of Human Lymphoid Tissue and Female Genital Mucosa with Human Immunodeficiency Virus 1 and Histoculture
11:14

Ex Vivo Infection of Human Lymphoid Tissue and Female Genital Mucosa with Human Immunodeficiency Virus 1 and Histoculture

Published on: October 12, 2018

HIV expression in surgical specimens.

Jan M Orenstein1

  • 1George Washington University Medical Center, Washington, DC 20037, USA. jorenstein@mfa.gwu.edu

AIDS Research and Human Retroviruses
|August 2, 2008
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The transcriptional profile of coronary arteritis in Kawasaki disease.

BMC genomics·2015
Same author

An evaluation of the validity of the animal models of Kawasaki disease vasculopathy.

Ultrastructural pathology·2014
Same author

Kawasaki Disease has so much to teach us!

Ultrastructural pathology·2014
Same author

Clinical implications of a new model of Kawasaki disease arteriopathy.

Pediatric cardiology·2013
Same author

Integrins α4 and αM, collagen1A1, and matrix metalloproteinase 7 are upregulated in acute Kawasaki disease vasculopathy.

Pediatric research·2013
Same author

Modulation of innate host factors by Mycobacterium avium complex in human macrophages includes interleukin 17.

The Journal of infectious diseases·2012

HIV RNA and Gag p24 protein expression were found in gastrointestinal lymphoid tissue, deep lymph nodes, and inflammatory lesions of HIV-infected patients. Viral expression levels were comparable to superficial lymph nodes.

Area of Science:

  • Virology
  • Immunology
  • Gastroenterology

Background:

  • Human Immunodeficiency Virus (HIV) establishes persistent infection.
  • Understanding viral reservoirs in various tissues is crucial for eradication strategies.

Purpose of the Study:

  • To quantify HIV-1 RNA and Gag p24 protein expression in gastrointestinal tract-associated lymphoid tissue (GALT), deep lymph nodes (LNs), and inflammatory lesions.
  • To compare viral expression levels across different anatomical sites and tissue types.

Main Methods:

  • Immunohistochemistry (IHC) for Gag p24 protein.
  • In situ hybridization (ISH) for HIV-specific RNA.
  • Transmission Electron Microscopy (TEM) for viral particle identification.

Main Results:

More Related Videos

Single-cell Quantitation of mRNA and Surface Protein Expression in Simian Immunodeficiency Virus-infected CD4+ T Cells Isolated from Rhesus macaques
13:13

Single-cell Quantitation of mRNA and Surface Protein Expression in Simian Immunodeficiency Virus-infected CD4+ T Cells Isolated from Rhesus macaques

Published on: September 25, 2018

Related Experiment Videos

Last Updated: Jul 3, 2026

Ex Vivo Infection of Human Lymphoid Tissue and Female Genital Mucosa with Human Immunodeficiency Virus 1 and Histoculture
11:14

Ex Vivo Infection of Human Lymphoid Tissue and Female Genital Mucosa with Human Immunodeficiency Virus 1 and Histoculture

Published on: October 12, 2018

Single-cell Quantitation of mRNA and Surface Protein Expression in Simian Immunodeficiency Virus-infected CD4+ T Cells Isolated from Rhesus macaques
13:13

Single-cell Quantitation of mRNA and Surface Protein Expression in Simian Immunodeficiency Virus-infected CD4+ T Cells Isolated from Rhesus macaques

Published on: September 25, 2018

  • HIV RNA and p24 protein were detected in GALT germinal centers (GC) on follicular dendritic cell (FDC) networks, comparable to LNs.
  • Deep LNs showed viral expression levels equivalent to superficial LNs.
  • Virus-expressing mononuclear cells (MNCs) were present in GALT, LNs, and inflammatory lesions like ulcers and appendicitis.
  • Abundant virus was observed in the cervix and LNs of cancer patients.

Conclusions:

  • GALT, inflammatory lesions, and deep LNs serve as significant sites for HIV expression.
  • Viral expression in these tissues is comparable to more commonly studied superficial LNs.
  • These findings highlight the widespread distribution of HIV reservoirs throughout the body.