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Scanning Electron Microscopy of Macerated Tissue to Visualize the Extracellular Matrix
10:21

Scanning Electron Microscopy of Macerated Tissue to Visualize the Extracellular Matrix

Published on: June 14, 2016

Extracellular matrix turnover and inflammatory markers independently predict functional status and outcome in chronic

Anca Radauceanu1, Camille Ducki, Jean-Marc Virion

  • 1Centre d'Investigation Clinique 9501, INSERM, Dommartin-lès-Toul, France.

Journal of Cardiac Failure
|August 2, 2008
PubMed

Insights

Markers of extracellular matrix (ECM) remodeling, specifically amino-terminal propeptide of collagen III (PIIINP) and matrix metalloproteinase 1 (MMP-1), predict functional capacity and outcomes in chronic heart failure (CHF) patients. These findings highlight ECM turnover

Area of Science:

  • Cardiology
  • Biomarker Research
  • Heart Failure Pathophysiology

Background:

  • Inflammatory pathways are implicated in extracellular matrix (ECM) remodeling and chronic heart failure (CHF) progression.
  • The interplay between inflammation, ECM remodeling markers, and CHF patient outcomes requires further investigation in large cohorts.

Purpose of the Study:

  • To examine the relationship between serum markers of inflammation and ECM remodeling.
  • To determine the influence of these markers on functional status and clinical outcomes in CHF patients.

Main Methods:

  • Serum levels of ECM markers (PIIINP, MMP-1, TIMP-1) and inflammatory markers (hsCRP, IL-18, IL-10) were measured in 1009 CHF patients from the RECOVER trial.
  • Correlations between marker classes were analyzed.
  • Multivariable models assessed independent predictors of the 6-minute walk test (6-MWT) and CHF-related mortality/hospitalization.

Main Results:

  • Positive correlations were observed between ECM remodeling markers and inflammatory markers.
  • Amino-terminal propeptide of collagen III (PIIINP) and matrix metalloproteinase 1 (MMP-1) independently predicted 6-MWT performance.
  • PIIINP was the sole independent predictor of death and CHF hospitalization.

Conclusions:

  • Excessive ECM turnover, indicated by elevated PIIINP and MMP-1, is associated with reduced functional capacity in CHF.
  • PIIINP is a significant independent predictor of adverse clinical outcomes, including mortality and hospitalization, in CHF patients.
Abstract

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